Respuesta :
In the DNA isolation process, "meat tenderizer" is used to break down the protein complexes and allow the DNA molecules to easily precipitate.
What is DNA isolation/extraction?
Extraction of DNA is a common practice used to separate DNA from cell nuclei.
The process of DNA isolation involves-
Step 1: Cracking open cells to release DNA
The cells in a specimen are separated from one another, frequently by physical techniques like grinding or vacuum filtration, and then placed in a salt solution.
Step 2: Distinguishing DNA from protein and additional cellular waste
It's important to get rid of as much cellular waste as you can to obtain a pure DNA sample. There are numerous ways to accomplish this. To destroy DNA-associated protein and other cellular proteins, a protease (protein enzyme) is frequently introduced.
Step 3: Use an alcohol to precipitate the DNA
Finally, the DNA sample is carefully infused with ice-cold alcohol (whether ethanol or isopropanol). While salt and alcohol make DNA insoluble, it is soluble in water.
Step 4: DNA cleaning
Now that the DNA sample has been refined further (cleaned). Then it is ready for use after being resuspended in such a slightly alkaline buffer.
Step 5: Verifying the DNA's presence and condition
The concentration of DNA in a sample can be ascertained using spectrophotometer readings of optical density.
To know more about process of DNA isolation/extraction, here
https://brainly.com/question/18316109
#SPJ4
