It is a thermodynamically spontaneous process that means Protein folding occurs. The value of ΔG must be negative in the case of folding but in the case of unfolding, the value of ΔG must be positive.
When the process is thermodynamically spontaneous process results in Protein folding otherwise the process is unfolding.
The value for ΔG must be negative in the case of the folding process and the value for ΔG must be positive in the case of the unfolding process. The value of a thermodynamic parameter for the reverse reaction has an opposite sign but the same value that's why the value for ΔG must be positive and known as unfolding (denaturation) process.
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