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The image that has been given in the context that does not comprise of the middle C is the Option B.

What is known as musical notes?

These are music that comprises several pitches that together make up the standard note of the music.

Here, the chromatic scale is used by the majority of musicians and involves seven main musical notes in the chromatic scale: A, B, C, D, E, F, and G.

Each of them represent a different pitch or frequency. The "middle" A note, for example, has a frequency of 440 Hz, whereas the "middle" B note has a frequency of 494 Hz.

Also, the sharp & flat notes are two variations of each of these notes. A half step up is a sharp, and a half step down is a flat. The C-sharp, for example, is a half-step up from C.

Therefore, the Option B is correct.

Read more about the musical notes

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