The hydrolysis of phosphatidylinositol 4,5‑bisphosphate (pip2) by phospholipase C generates inositol 1,4,5-trisphosphate ( [tex]IP_{3}[/tex] ) and diacylglycerol (DAG) secondary messengers.
The small molecules and ions relay signals received by cell-surface receptors to effector protein is referred as second messenger.Examples of secondary messengers are cyclic AMP,cyclic GMP inositol 1,4,5-trisphosphate ( [tex]IP_{3}[/tex] ) ,diacylglycerol (DAG) ,and calcium. [tex]IP_{3}[/tex] induces a transient increase in intracellular free [tex]Ca^{+2}[/tex] ,whereas DAG directly activates protein kinase C.
There are two distinct kinds of signaling pathways that use phosphatidylinositol. Phospholipase C breaks down phosphatidylinositol-4,5-bisphosphate to create IP3, which serves as a crucial part of the Ca-signaling mechanism described earlier.
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