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If an organism is catalase positive, it is in staphylococcus aerotolerance categories.

Catalase testing is used to distinguish between Streptococci (catalase-negative) and staphylococci (catalase-positive). The catalase enzyme works to counteract hydrogen peroxide's bactericidal effects. Hydrogen peroxide (H2O2) is quickly broken down into water and oxygen by catalase.

Bacteria that use oxygen for breathing create the enzyme catalase, which shields them from the harmful byproducts of oxygen metabolism. Strict aerobes and facultative anaerobes are both catalase-positive bacteria, although they can all respire utilising oxygen as a terminal electron acceptor. Anaerobes or facultative anaerobes that solely ferment and do not respire using oxygen as a terminal electron acceptor can be catalase-negative bacteria (i.e. Clostridium).

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