The original "one gene–one enzyme" hypothesis was modified to " one gene–one polypeptide" because not all proteins are enzymes, and many proteins are built from two or more distinct polypeptide chains.
According to the one gene, one enzyme hypothesis, which was put forth by George Wells Beadle in the US in 1941, each gene directly generates a single enzyme, which then influences a particular stage in a metabolic pathway. Since one gene can produce several proteins and not all proteins are enzymes, the one gene, one enzyme hypothesis of Beadle and Tatum has been amended. We also know that enzymes are made up of many polypeptides. Therefore, "one gene-one polypeptide" has been changed to make the hypothesis more precise.
In contrast to the one gene-one polypeptide hypothesis, which claims that one gene regulates the creation of just one polypeptide chain of an enzyme molecule, the one gene-one enzyme hypothesis states that one gene controls the production of one enzyme.
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