performing a endospore stain on a gram-positive bacteria and that is not endospore forming. at the end of the stain it will look pink due to absence of any endospores.
- Some bacteria create bacterial endospores, extremely resistant, metabolically dormant entities as a defense mechanism against unfavorable environmental conditions.
- Until conditions are right, the bacteria can stay in this suspended state and eventually germinate and go back to their vegetative state.
- By heating the bacterial emulsion, the Schaeffer-approach Fulton's forces a primary stain, malachite green, into the spore.
- Vegetative cells may be decolored with water since malachite green is water soluble and has a poor affinity for cellular matter.
- After that, safranin is used to counterstain any decolored cells. Vegetative cells will be pink and endospores will be a dark green color after staining.
- However due to absence of endospores, the counterstain will not produce any distinguish features and the slide would appear pink.
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