Because of the ability to identify and quantify proteins in a complex mixture, mass spectrometry combined with separation methods is an excellent analytical tool used specifically in the field of Proteomics.
- Proteomics based on mass spectrometry is now the method of choice for identifying and measuring an organism's proteome.
- There are many chances for statisticians to make substantial contributions, even if recent years have seen a dramatic advance in instrument performance and the computational techniques employed.
- The most popular "bottom-up" method for proteomics involves enzymatic cleavage of complex protein mixtures, followed by the separation of the peptide products based on their physical or chemical characteristics and mass spectrometric analysis.
- Identifying the proteins that are present in a sample and quantifying the abundance levels of the detected proteins are the two main difficulties in the analysis of bottom-up MS-based proteomics.
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