RNAi is a gene silencing technique that is based on the fact that RNA molecules can fold into short, double-stranded regions where the base sequence is complementary.
A conserved biological response to double-stranded RNA, known as RNA interference (RNAi) or post-transcriptional gene silencing (PTGS), promotes resistance to both endogenous parasites and exogenous harmful nucleic acids and controls the expression of protein-coding genes. Small double-stranded RNA (dsRNA) molecules are used as triggers by most eukaryotic cells' RNA interference (RNAi) regulatory mechanisms to direct homology-dependent control of gene activity.
In order to protect plants from molecular parasites like viruses, RNA silencing has evolved. In controlling gene expression for growth and development, it is also helpful. These pathways interact with one another and generate short RNAs to function.
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