Ribulose 1,5-biphosphate will be depleted while 3-phosphoglycerate will accumulate.
The first step of the carbon fixation during which Rubisco adds CO2 to Ribulose 1,5-biphosphate to produce 2 molecules of 3-phosphoglycerate requires energy molecules from the light reaction.
In the subsequent steps, Ribulose 1,5-biphosphate is regenerated in a step that requires energy molecules from the light reaction.
Thus, in the first few minutes of turning off the light, the available energy molecule from the light reaction will be used up to produce 3-phosphoglycerate. However, the regeneration of Ribulose 1,5-biphosphate will become impossible.
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