The sequence of the DNA template is ACCTT. This is because a five-base read from a sequencing reaction produced in the color sequence, read by the computer such as red, yellow, yellow, green, and green.
DNA template may be defined as the strand that is utilized during transcription to construct RNA. It is complementary to the target strand of the DNA.
ddNTPs are labeled with the fluorescent dyes specifically like ddATP is labeled with the red fluorescent dye, ddTTP is labeled with the green fluorescent dye, ddCTP with the yellow, and ddGTP with the blue. The sequence that is synthesized during the sequencing reaction possesses colors like red, yellow, yellow, green, and green.
Therefore, the sequence of the DNA template is ACCTT.
To learn more about Sanger's method of DNA sequencing, refer to the link:
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