Phylogenetic inference is the practice of reconstructing the evolutionary history of related species by grouping them based on shared ancestry.
If this organism belongs to vertebrates then the notochord is replaced with a spinal cord. This difference is the main feature that separates invertebrate from vertebrate with a backbone.
So we can conclude that presence of backbone is the main difference between invertebrate and vertebrate organism.
Learn more about invertebrates here: https://brainly.com/question/10006169