If, while examining a human karyotype, it is observed that there are 22 paired chromosomes and two chromosomes that are not the same size, this would most likely be due to mutation.
Mutation is any change in the nucleotide or chromosomal sequence of a genome.
There are different types of mutation and they include;
According to this question, a human karyotype was observed to have 22 paired chromosomes and two chromosomes that are not the same size.
This observation is likely caused by a mutation that caused the pairing of unequal sized chromosomes.
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