Question

7. Find the restriction sites and "cut" the DNA in the sequence below. How many bands of DNA would
you see on the electrophoresis gel?

BamI (CCTAGG) --- 5' CCTAGG 3'; EcoRI (GAATTC) --- 5'G LAATTC 3'

5'ACGAATTCAGTATTATCCTAGGTATCCGCCGCCGAATTCTCATCA 3
3’TGCTTAAGTCATAATAGGATCCATAGGCGGCGGCTTAAGAGTAGT 5

Answer 1

Based on their recognition sequences, two DNA bands will be produced by Bam1 and three DNA bands will be produced by EcoR1.

What are restriction sites?

Restriction sites are sequences of nucleotides which are recognized by restriction enzymes and are acted upon by the restriction enzymes.

Restriction enzymes cuts DNA at recognition sites based on their recognition sequences.

Examples of restriction enzymes are Bam1 and EcoR1.

For Bam1, the recognition sequence is (CCTAGG) --- 5' CCTAGG 3'

Two bands will be produced using Bam1 as shown below:

5'ACGAATTCAGTATTATCCTAGG 3'

3'TGCTTAAGTCATAATAGGATCC 5'

5'TATCCGCCGCCGAATTCTCATCA 3'

3'ATAGGCGGCGGCTTAAGAGTAGT 5'

For EcoR1, the recognition sequence is (GAATTC) --- 5'GAATTC 3'

Three bands will be produced using with EcoR1 as shown below:

5'ACGAATTC 3'

3'TGCTTAAG 5'

5'AGTATTATCCTAGGTATCCGCCGCC 3'

3'TCATAATAGGATCCATAGGCGGCGG 5'

5'TCATCA 3'

3'AGTAGT 5'

Therefore, two DNA bands will be produced by B-am1 and three DNA bands will be produced by Eco-R1.

Learn more about restriction sites at: https://brainly.com/question/8886948