What is the recommended minimum temperature setting for an autoclave to inactivate biohazards, including spores, if it is operated for the appropriate contact time?.

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The recommended minimum temperature setting for an autoclave to inactivate biohazards is 121 ºC. It is fundamental to avoid contamination.

Autoclave and contamination

An autoclave is a device used in molecular biology laboratories to avoid cross-contamination.

This laboratory tool (autoclave) is a pressure chamber that functions under elevated temperatures and pressure.

An autoclave can be used to inactivate (kill) different types of pathogenic microorganisms (e.g. bacteria).

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