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Cut two leafy shoots of balsam plant (FIGURE) under water to prevent entry of any air bubble. Now by keeping lower end of shoot in water remove near about 2-3 cm outer ring (phloem) of stem, keeping the central part intact. In second shoot, remove the central part (xylem) of equal length. Now fix both the shoots in stands and observe, after two days we find that the leaves of first shoot remain turgid and stands normally, but in second twig leaves wilt and droop down, this experiment proves that water is conducted through xylem.

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