Question

The Sanger method of DNA sequencing uses a mixture of dNTPs and ddNTPs, which are missing oxygen atoms at both the 2' and 3' carbons. What is the role of the ddNTPs?


A.

They stop replication because they are not able to bind to the growing DNA strand.


B.

They stop replication because the next nucleotide in the growing strand cannot bind to them.


C.

The enhance replication because DNA polymerase selectively adds them to the growing DNA strand.


D.

They enhance replication because they form additional hydrogen bonds with the complimentary DNA strand.

Answer 1

The role of the ddNTPs is to stop replication because the next nucleotide in the growing strand cannot bind to them. It is for that reason that ddNTPs are fundamental in Sanger sequencing.

Sanger sequencing

Sanger sequencing is a technique used to sequence and thus determine the nucleotide order of a DNA chain.

This methodology (Sanger sequencing) was designed by Frederick Sanger in 1977.

In Sanger sequencing, the ddNTPs are used to stop the synthesis of a DNA strand due to the lack of a free hydroxyl group (OH) which is required for the replication of DNA.

Learn more about Sanger sequencing here:

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