The order for rDNA is to identify a target gene, select a plasmid, use a restriction enzyme, gene insertion, use ligase, introduce the recombinant DNA and detect recombinant cells. rDNA involves genetic recombination.
Recombinant DNA (rDNA) technologies are used in molecular biology laboratories to create chimeric DNA molecules containing nucleotide sequences from different species.
Recombinant DNA techniques can be used to identify and sequence genes, as well as to discover gene function.
The sequential steps of rDNA technologies are as follow:
- Identify a gene of interest.
- Select a DNA plasmid to be used.
- Use restriction enzymes to cleave the plasmid DNA.
- Introduce the gene of interest in the plasmid.
- Use ligase enzymes to join both nucleotide sequences.
- Introduce the recombinant DNA molecule into the host cell.
- Detect and replicate the cells containing the desired recombinant DNA molecule.
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