now you’ll examine an operon that undergoes negative and positive control. the ara operon in e. coli codes for enzymes that metabolize the sugar arabinose, which e. coli can use for energy. as you can see in the diagram, the regulatory protein arac can act as both an activator and a repressor of the ara operon.

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The lac operon is known to be able to undergoes negative and positive control.

The diagram based on the 4 table arrangement is filled up below (Right to left);

  • In the presence of arabinose:  initiator, activator,  yes,  yes.

  • In the absence of arabinose: initiator and operator, repressor, no, no.

  • If a mutation prevents arabinose from biding to AraC: initiator and operator, repressor, no, no.

  • If arabinose is present but a mutation and AraD enzyme renders it non-functional: initiator, activator, yes, no.

The metabolism of the sugar arabinose is known to be influenced  by an operon that is said to undergo both positive control and negative control.

Transcription of the ara operon is lead by the regulatory protein AraC, which then bound to the DNA.

When arabinose is present, it binds to AraC, and both parts of the AraC dimer are then also bound to the initiator, making transcription to move foreword.

When arabinose is absent, one part of the AraC dimer is often bounded to the initiator, while the other part is bounded to the operator, thereby hindering transcription.

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Now you'll examine an operon that undergoes negative and positive control. The ara operon in E. coli codes for enzymes that metabolize the sugar arabinose, which E. coli can use for energy. As you can see in the diagram, the regulatory protein AraC can act as both an activator and a repressor of the ara operon.

Use the diagram above to fill in the table.

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