Respuesta :
Answer:
a. It is important to isolate the undigested plant material since the digested plant material cannot be used since the cell wall will be broken and the enzymes like DNases and other digestive enzymes will break down the organelle membranes and the DNA will be degraded.
If it is not isolated properly the other contaminants and the other enzymes, proteins and debris will interfere with the quality of the DNA. The isolation of undigested ensures that intact DNA can be isolated with the minimum amount of unwanted contaminants.
b. Metabarcoding is the technique through which several DNA/RNA samples are isolated and sequenced from a small or section of the sequence from a common sample to identify the different species present. This is done by isolation, amplification (PCR), and sequencing using a sequencer by comparing to the database. The database is formed by sequencing the known species in the area and then comparing the sequence data obtained from the sample to the database and when it matches the database sequence we can conclude that this species is present in the sample. Hence DNA from 400 plant species that grow on the African savanna and entry of the sequence data into a database was important.
Explanation:
