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A reference to estimate the size of unknown DNA molecules that were separated based on their mobility in an electrical field through the gel.

The function of a ladder in gel electrophoresis is to gauge the size of bands in the sample. Thus, option A is correct.

What is ladder in gel electrophoresis ?

Gel electrophoresis is a method of visualization, separation, purification, isolation of biological molecule like DNA, RNA, proteins based on  their size.

After supplying electric charge to electrophoresis tray, the negatively charged DNA molecules moves to positive pole and smaller DNA molecules  can move faster than large sized molecule.

A ladder is a standard weight-sized artificially made reference molecular marker which is used to determine the size of the unknown DNA fragment.

RNA ladder and protein ladder are used in terms RNA and protein related experiments.

DNA ladders varies from different lengths with known fragment size and concentration

Hence, option A is correct.

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Your question was incomplete. So the probable question was

what is the function of a ladder in gel electrophoresis?

A. Gauge the size of bands in the sample

B. Prevent the bands from running of the gel

C. Negative control

D. positive control