The following formula is useful to determine the size of the cell ⇒ Size of the object = FOV / Fit number = dFOV / Fit number. If you have this data, you can estimate the size of the onion cell. According to the values we used, the estimated size of this onion cell is 112 μm.
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Cells are too small to measure their sizes, so to know their actual size, we can make mathematical estimations to know their sizes, or we can use software programs. In this case, we are estimating their size using the following formula,
Size of the object = FOV / Fit number = dFOV / Fit number
- Size of the object = size of the cell
- FOV = eyepiece (mm)/objective magnification
- Fit number = The number of times the object -cell- fits in the field of view
To answer this question, we will replace the terms in the given formula.
→ First, we will get the FOV value. From references, we can assume that
- under 4X ⇒ FOV = 4.5 mm
- under 10X ⇒ FOV = 1.8 mm
- under 40X ⇒ FOV = 0.45 mm
You can also calculate the FOV by using a millimeter paper or rule.
Note: We will use these values to replace terms in the formula, but you can use the dFOV value calculated in question no. 3.
→ Second, we will count the number of cells that fit the field. We can see about four cells across the field of view.
→ Finally, we will replace the terms in the formula.
Size of the object = FOV / Fit number
Size of the object = 0.45 mm / 4
Size of the object = 0.112 mm
Since we generally use μm when referring to cell sizes, we need to multiply 0.112 x 1000 to get the size of the cell expressed in μm.
Size of the cell (μm) = 0.112 mm x 1000 = 112 μm
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