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28-05-2021
Biology

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Getting a cloned eukaryotic gene to function in bacterial host cells can be difficult. What are two problems with bacterial gene expression systems, and how is each solved

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28-05-2021

Answer:

certain aspects are different in eukaryotes and bacteria- expression vector is a cloning vector that contains a highly active bacterial promoter just upstream of a restriction site

expressing cloned eukaryotic genes in bacteria is the presence of non coding regions- using cDNA

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