In an experiment to study the effect of amylase on starch, samples of substrate and enzyme solutions are taken. The
volumes of substrate and enzyme solutions in each sample are the same. The temperature at which the experiment is
performed is also constant. The buffer solutions used for the experiment have pH 3. pH 5. pH 7. pH 9. and pH 11.
What can be BEST predicted about the result?

A. the results would be consistent because amylase activity is independent of pH

B. the results would be consistent at all pH values because there would be no breakdown of starch

C. the results would vary because the active sites of amylase in each sample are different

D. the results would vary because an increase in pH increases the formation of this produce