Answer:
- A large number of non-protein coding sequences are transcribed
- Total transcription changes over time in embryonic stem cells
Explanation:
The transcriptome refers to the totality of RNA molecules (i.e., mRNAs, microRNAs, long noncoding RNAs, etc.) that are synthesized in a given cell type/tissue/development stage. The analysis of human transcriptome has been essential to discover genes and non-coding RNAs expressed at each developmental stage, as well as genes whose expression is associated with human diseases. Next-Generation Sequencing (NGS) information showed that more than 90% of the human genome is transcribed into RNA, especially non-protein-coding sequences (i.e., non-coding RNAs). Moreover, information on the human transcriptome evidenced that global transcription changes occur in pluripotent embryonic stem cells, and these changes are mainly associated with the expression of chromatin-remodeling genes as well as genes responsible to encode the components of the general transcription machinery.
