A lawn of Pseudomonas aeruginosa was grown for 24 hours and then scraped completely. The mass of bacteria was suspended in 5ml of sterile saline. An aliquot of the suspension was measured using a Spectrophotometer.
The absorbance or OD600 of the initial suspension was measured at 0.892.
The suspension was serially diluted. In each tube 1ml of the previous dilution was added to 9ml of saline. In the boxes above the tubes write the dilution of that tube as a ratio. The first one is done for you.
