Question

To ensure that a human gene is translated properly in a bacterial cell, you should clone the _____________ for the gene instead of the genomic DNA fragment that contains the gene.

Answer 1

Answer:

complementary DNA (cDNA)

Explanation:

The complementary DNA (cDNA) is a region of DNA that is synthesized from an RNA template by using a reverse transcriptase enzyme. cDNA is usually used to clone genes from eukaryotic organisms in prokaryotes. cDNA cloning is a technique that consists of isolating and amplifying a self-replicating prokaryotic organism that includes in its genome a cDNA of interest. This technique enables the insertion of fragments of eukaryotic DNA (cDNA) into plasmids that subsequently are multiplied by cloning and finally sequenced.