Answer:
b. Forward or reverse primers
Explanation:
Sanger sequencing is a technique of DNA sequencing based on the extension of DNA fragments with variable sizes terminated with dideoxynucleotides at the 3′ end. This technique was developed by Frederick Sanger in 1977. In Sanger sequencing, a short primer is added in order to bind by complementarity to the target DNA region of interest. Subsequently, a DNA polymerase adds nucleotides (A, T, C and G) in the 5'-3' direction. Finally, the extension of the DNA strand is stopped by adding dideoxynucleotides, which are nucleotide analogs (i.e., modified nucleotides) that act as DNA synthesis terminators.
When setting up a Sanger sequencing reaction, each reaction should include template DNA, nucleotides, dideoxynucleotides, buffer, DNA polymerase, and forward or reverse primers.
Sanger sequencing refers to a laboratory procedure that's used in the determination of DNA sequence by using dideoxynucleotides. Sanger sequencing is also known as dideoxy sequencing.
For Sanger sequencing to work, one needs two pieces of information that will be used to apply the strategy of sentence game to the sequencing of DNA. It should be noted that forward or reverse primers are required for the reaction.
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