Answer: The question is incomplete and the options are not given, from my searches I was able to get the completed part which are;
A. Antibodies can be used to detect the antigen on a western blot
B. Antigens can be centrifuged through a column and pellet at specific speeds C. Antigens can easily be purified by charge and size when coated with SDS D. Antigens will separate by size going through the column beads and their holes
E. Organelles containing the antigen will have a specific buoyancy and separate in a gradient.
The correct option is D.
Antigens will separate by size going through the column beads and their holes
Antibodies can be used to detect the antigen on a western blot
Explanation:
The best method for purifying an antigen for injection into a mouse to generate monoclonal antibodies by gel filtration chromatography is Antigens will separate by size going through the column beads and their holes because Gel filtration chromatography is a size exclusion chromatography that work on the principle that molecules are separated based on their big sizers or larger sizes. The columns are designed to remove substances that are smaller in sizes. The larger size antigen elute first.
