In this case, Lines 5, 4 and 1 represent the same species as Line 8 in the electrophoresis gel.
What is an electrophoresis gel?
An electrophoresis gel is a device used to separate molecules according to their charge and molecular weight.
Electrophoresis can be used to identify DNA samples due they may have PCR bands may have different sizes.
In conclusion, in this case, Lines 5, 4 and 1 represent the same species as Line 8 in the electrophoresis gel.
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