The lungfish Protopterus aethiopicus has a genome 38 times larger than that of humans. Most of the DNA in this species is noncoding repetitive DNA. What type of library would allow you to compare just the genes in the lungfish to the genes in humans? Select one: a. PCR library b. cDNA library c. Transgenic library d. Genomic library e. Knockout library

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Answer:

cDNA library

Explanation:

The complementary DNA (cDNA) library is a library of genes of an organism that can be encoded into a functional protein, meaning it excludes all non-coding repetitive DNA. The complementary DNA can be made by the process of reverse transcription of the messenger RNA (mRNA), by the enzyme reverse transcriptase to form a DNA transcripts. These DNA transcripts can now be cloned to form the cDNA library.

Owing to the fact that the genome of human is 38 times less than that of lung fish, with that of lung fish having a lot of noncoding repetive DNA, the best way to compare their genes is by the use of cDNA library, because it only contains genes that codes for a protein and hence helping to exclude the non coding sequence of lungfish.

The answer is cDNA library

A cDNA library is a group of replicated DNA sequences that are equivalent to the mRNA that was isolated from an organism or tissue.

What is cDNA library?

  • The easiest technique to compare the genomes of these fish and humans is through a comparative analysis of their coding sequences, particularly those of protein-coding genes.
  • The cDNA library, which is made up entirely of protein-coding sequences, is created when all of an organism's mRNAs are collected, reverse-transcribed into DNA copies, and then cloned into the appropriate genetic vector.
  • Since a significant portion of the library's non-coding sections are removed during the reproduction of eukaryotic genomes, cDNA libraries are frequently employed.
  • Eukaryotic genes are expressed in prokaryotes using cDNA libraries.

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