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In the transition of metaphase to anaphase, the cohesin complex is cleaved by the separase enzyme in a process dependent on the activation of specific proteins that trigger posttranslational modifications (i.e., protein degradation by ubiquitination). This process of cleavage enables the sister chromatids to separate and move to opposite sides of the cell
- A model for how bivalent DNA binding of the cohesin complex (consisting of Smc1, Smc3, Scc1 and Scc3) might hold sister chromatids together during metaphase. At anaphase, separase recognizes and cleaves two distinct sites in the Scc1 subunit.
- In the transition of metaphase to anaphase, the cohesin complex is cleaved by the separase enzyme in a process dependent on the activation of specific proteins that trigger posttranslational modifications (i.e., protein degradation by ubiquitination). This process of cleavage enables the sister chromatids to separate and move to opposite sides of the cell.
Thus, In the transition of metaphase to anaphase in mitosis is the point at which separase cleaves the protein complex that holds sister chromatid pairs together.
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