Nucleotides can be radiolabeled before they are incorporated into newly synthesized DNA and, therefore, can be assayed to track their incorporation. In a set of experiments, a student-faculty research team used labeled T nucleotides and introduced these into a culture of dividing human cells at specific times. Which of the following questions might be answered by using the method described?
A. When do spindle fibers attach to chromosomes?
B. How many picograms of DNA are made per cell cycle?
C. What is the length of the S phase of the cell cycle?
D. How many cells are produced by the culture per hour?

Such type of technique that is used to tack or identified the passage or location of the metabolic reaction, organelles and some other during the research or experiment is known as radiolabelling.
During the study of the synthesis of the DNA, the nucleotides are labeled to check the S phase of the cell cycle.
Since the synthesis of the DNA takes place during this phase so the time and length of the S phase can be easily obtained during the experiment.
This technique is also used for different purposes during the biomolecular experiment and some other disciplines.

oeerivona oeerivona · Answer 2 · 2021-11-24T22:49:24+01:00

The S phase is the DNA replication phase of the cell cycle, it is the phase

that occur between the G₁ and G₂ phases.

The question that might be answered by using the method described is; D. What is the length of the S phase of the cell cycle?

Reasons:

The S phase, or synthesis phase, is the cell division phase in which the

DNA of the cells are replicated such that the number the genome is

doubled, before the cell enters into the meiosis mitosis stage.

The synthesis that takes place during the s-phase includes the synthesis of

nucleotides.

Therefore, the radiolabeled nucleotides can be incorporated in the s-phase

after the DNA is synthesized, and therefore, the length of the S-phase of

the cell cycle can be determined.

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