Question

You have recovered two LacZ-minus AmpR colonies from your transformation in Lab 7 and they appear as white colonies on a LBAX plate. You test each colony on a LBT plate. Only one of these two colonies grew on the LBT plate (Colony 1) while the other colony did not grow on the LBT plate (Colony 2). What is most likely the plasmid contained in Colony 2?
A: pUC119
B: pACYC184
C: pUC-tet(resistant)
D: pUC-chloramphenicol(minus)

Answer 1

Answer:

D. pUC-chloramphenicol(minus)

Explanation:

It contains chloramphenicol resistance gen, the PMB1 posses origin of replication (ori), beta-galactosidase coding gen Laz. It also has pUC18 with many cloning site in the Lac Z gene which makes the recombinant clones to be verified via culture plates which is made up of IPTG and X- Gal.