Question

A student finishes an experiment involving several bacteria which are highly pathologic in humans. She was to dispose of the agar plates and micropipette tips she used. Which of the following procedures should she carry out?

(A) Microwave all materials for more than 60 seconds.
(B) Wipe down on materials with 100% ethanol solution.
(C) Place all materials in a biohazard bag and autoclave the bag.
(D) Place all materials under UV light for 90 seconds.

Answer 1

Sterilization techniques

Explanation:

(C) Place all materials in a biohazard bag and autoclave the bag.

Firstly,Autoclaving is a wet sterilization method. In an autoclave laboratory materials can be sterilized at a temperature of 120 degree Celsius under 2 atm pressure.  Under these condition no cell could survive. Secondly bio-hazard bags are designed specially so that they could prevent the leak of such bio-hazards. So, to dispose off such pathogenic organism  by placing the petridishes in bio-hazard bag and autoclaving will be the best method.