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To assist in completing this question, you may reference the Animated Technique Video - Isoelectric Focusing. What characteristics determine the position of a protein on an IPG strip at the end of isoelectric focusing? a. the protein's three‑dimensional structure b. local pH in the medium c. protein solubility d. the pI of the protein e. the molecular weight of the protein

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Answer:

The correct answers are options b and d.

Explanation:

Both the local pH of the medium and the isoelectric point or pI of the protein are the features, which helps in determining the protein position on the IPG strip at the termination of the isoelectric focusing.  

It is known that proteins refer to the biomolecules that get produced from amino acids. Amino acids are the components that comprise both carboxylic as well as amino group, and in isoelectric point the movement between the amino acid ceases.  

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