The polymerase chain response (PCR) could be a strategy to quickly open up groupings of DNA.
Amid a normal PCR, format DNA (containing the locale of intrigued) is blended with deoxynucleotides (dNTPs), a DNA polymerase and small segments of DNA called primers.
PCR involves a series of temperature cycles.
The temperature used for the extension step is 72°C.
The temperature that is used during the extension phase is dependent on the DNA polymerase that is used.
Amid the expansion step (ordinarily 68°C-72°C) the polymerase amplifies the groundwork to create an early DNA strand. This prepare is rehashed different times
Generally at this stage, the reaction mixture is heated to a temperature intermediate between the denaturation and annealing temperatures. The optimal temperature for Taq polymerase is 72°C.
