Question

A student from one of the research labs is having trouble preparing a slide for examination and photographing. The bacterial slide that he has brought to you was prepared using a commercially purchased stain. He has asked for your help in determining what he is doing wrong so that he can change the lab protocols and continue on with his project. After examining the slide under oil immersion, you determine that no bacteria are present even though the student is able to show you the culture he used to make that slide that has visible growth in the liquid medium. Which of the following statements does NOT explain the fact that there are no bacteria present on the student’s slide?

Answer 1

Answer: Rinsing with alcohol during the washing step stripped the bacteria off the glass slide.

Explanation:

The step that is being wrong in the whole experiment is that the student is washing the bacteria on the slide with alcohol.

Alcohol might have washed off all the bacteria as it is corrosive in nature. The bacteria that is going to be observed in the experiment is being killed when it is washed off.

This was the wrong step opted by the student if he finds any alternative solution to this then the bacteria can be observed in the microscope.