Read the clinical scenario, and then answer the questions that follow to become familiar with the traditional NCLEX question format. Ms. Hungh, a Burmese immigrant, enters your clinic with her interpreter complaining of fatigue, weight loss, persistent cough, and rust-colored sputum The interpreter explains that Ms. Hungh has had this cough for many months in her home country and, now that she is in America, is seeking assistance for her condition. Culturing of the sputum resulted in the growth of distinct colonies on the medium, and the technician informs you that further isolation by subculturing is now needed. You understand that this is accomplished by taking a bit of growth from an isolated colony and inoculating a separate medium, resulting in the production of a:______.
a) diagnosis.
b) pure culture.
c) broth.
d) mixed culture.

Obtaining a pure culture involves culturing the organism on the bacteriologic agar. Initially blood agar is used because it supports the growth of many bacteria and the type of hemolysis can be observed.

In order to obtain pure cultures, following steps are done :

1)The bacteria are normally streak onto solid media.

2) Inoculating loops are used for streaking.

3) Then the plate is incubated at desired temprature.

4) Then after sometimes , colonies are visible wherever a microbial cell capable of growth on particular medium was deposited on the agar surface.

As in the above scenario , a bit of isolated colony is taken and is inoculated in a separate medium thus a pure culture is formed.

Hence option B) Pure culture is the right answer.