Question

Enzyme X exhibits maximum activity at pH = 6.9. X shows a fairly sharp decrease in its activity when the pH goes much lower than 6.4. One likely interpretation of this pH activity is that: Substantiate your answer with brief explanation for your positive and negative answers.

A) the reaction relies on specific acid-base catalysis.
B) a His residue on the enzyme is involved in the reaction.
C) a Glu residue on the enzyme is involved in the reaction.
D) the enzyme is found in gastric secretions.
E) the enzyme has a metallic cofactor

Answer 1

Explanation:

A) the reaction relies on specific acid-base catalysis.

Enzymes are proteins that catalyze reactions by acting on substrates to facilitate a breakdown of large polysaccharides, like amylase. Many are affected by pH, temperature, and inhibitor presence- these affect their specificity.

These can experience shifts in their physical structure, called denaturation, in temperatures outside their optimal range; when denatured, enzymes lose their ability to attach directly to their substrate -i.e. The substrate binding capacity is impaired.

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