Question

Using solid-phase synthesis, you want to synthesize a 70-mer oligonucleotide with an overall yield of at least 60%. Your gene machine has a coupling efficiency of 99%. Will you be able to achieve your goal? Show the math.

Answer 1

Answer:

No,The goal cannot be achieved

Explanation:

In calculating solid phase synthesis,

Yield(Y) = CE^n-1

CE is coupling efficiency

n is length of oligonucleotide

Therefore, according to the question

CE is 99%= 0.99(99/100)

n is 70

Y= 0.99^70-1= 0.99^69= 0.499

Converting to percentage

0.499 x 100=49.9% approximately 50%.