Question

Complete the description of Christian Anfinsen's ribonuclease A renaturation experiment Anfinsen started with purified ribonuclease A in its native state. To begin, he ____________This process resulted in denatured ribonuclease A protein. Next, he _______ which allowed ribonuclease A to return to its native state.

Answer 1

Answer:

Urea and mercaptoethanol, first removes urea then mercaptoethanol.

Explanation:

The Anfinsen determines that the primary sequence is responsible for the determination of the secondary and tertiary structure of the protein. The protein are held together by covalent as well as non covalent interaction.

The Anfinsen uses urea and mercaptoethanol for the protein denaturation. Beta mercaptoethanol reduces the disulphide bond interaction and urea disrupt the alpha helical structure of protein. The protein renature 1% only when he first remove mercaptoethanol and then urea. the protein renaturation is around 99% when he first removes urea and then mercaptoethanol.

Thus, the answer is urea and mercaptoethanol, first removes urea then mercaptoethanol.