Based on what you have learned about microtubule (MT) binding protein, you hypothesize that gamma-tubulin is sufficient to nucleate MTs at the centrosome. Experiments to test your hypothesis are made difficult by that fact that in the cells that you are using, MTs are also nucleated at the Golgi, which is positioned next to the centrosome. Thus, you cannot clearly distinguish if MTs are nucleated at the centrosome or the Golgi. You had a great idea that will allow you to test your hypothesis. Which of the following experiments will be most useful to test your hypothesis?A) Remove gamma-tubulin by the CRISPR gene knockout method and monitor tubulin organization by live imaging
B) Express a mutant version of gamma tubulin that is fully function and which associates with mitochondria. In these cells, monitor MT organization in fixed cells
C) Overexpress gamma-tubulin and monitor the organization of the ER
D) Express a mutant version of gamma tubulin that is fully functional and which associates with the Golgi. In these cells, monitor the organization of MTs
E) Depolymerize MTs with the drug nocodazole and observe ER organization
