Question

A protein subunit from an enzyme is part of a research study and needs to be characterized. A total of 0.160 g of this subunit was dissolved in enough water to produce 2.00 ml of solution. At 28 ∘c the osmotic pressure produced by the solution was 0.138 atm. What is the molar mass of the protein?

Answer 1

Answer:

The Molar Mass (MM) of the protein is 14318.5g/mole

Explanation:

Number of moles (n) of the protein = mass/MM = 0.16/MM

From the ideal gas equation

PV = nRT

P = 0.138 atm, V = 2ml = 2cm^3, n = 0.16/MM mole, R = 82.057 cm^3.atm/gmol.K, T = 28°C = 28+273 = 301K

0.138 × 2 = 0.16/MM × 82.057× 301

MM = 3951.9/0.276 = 14318.5g/mole