Answer:
The four major methods of separation and purification techniques in the laboratory are as follows,
1. Chromatography
2. Recrystallization,
3. Distillation, and
4. Extraction
Spectroscopy however is not a common separation and purification method
Explanation:
Mass spectrometer separates ions in respect to the ratio of their mass to the their charge and are then detected based on their relative abundance this technique is used in the analysis of biomolecules