Select the four TRUE statements below describing the standard free energy change (ΔG) and the equilibrium constant (Keq).
Options:
1. The standard free energy change is a function of enzyme concentration in the reaction.
2. The value for the equilibrium constant can be experimentally determined for a given reaction.
3. The equilibrium constant is equal to ratio of the concentration of reactants over products at equilibrium.
4. The value for the standard free energy change is constant for a given reaction.
5. The equilibrium constant is the ratio of product and reactant concentrations at equilibrium.
6. If the value of the equilibrium constant is known, the value for the standard free energy change can be determined.
7. A reaction is spontaneous when the standard free energy change and the equilibrium constant are both negative values.

Explanation: Equilibrium constant is the ratio of the concentration of product and reactants. Its value just depend on the temperature. Thus the value for the equilibrium constant can be experimentally determined for a given reaction .

If the value of the equilibrium constant is known , one can easily determine the value of the gibbs free energy. Thus the value for the standard free energy change is constant for a given reaction.

A reaction is spontaneous only when the standard free energy has aa negative value, Equilibrium constant can never have the negative value.

The standard free energy change is a not function of enzyme concentration in the reaction.