The longer an analyte remains on the column, the broader the peak becomes. Peaks widen with time on column due to: A. viscosity. B. adsorption. C. absorption. D. diffusion. E. effusion.

Peaks, in a column chromatography, widen with time on column due to adsorption of the elements of the mixture to the solid matrix of the stationary phase.

Explanation:

Column chromatography uses a solid stationary phase and liquid mobile phase in the separation of an aqueous mixture. The resolution of the separation depends on the right flow rate/retention times of the different elements in the mixture. This is influenced by differential adsorption rates of the different elements to the solid matrix of the stationary phase hence eluting at different times.

The rate of adsorption should not be too high nor too low for a good resolution. The peak shape of the elutes should determine how well the resolution is. The peaks should not be too broad (where very string adsorption reduced the flow rate) because they will cause overlapping of peaks and hence reduce the resolution of the separation.

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