Answer:
Citrate synthase, acetyl-CoA
Explanation:
Beta oxidation is the process by which fatty acid molecules are broken down in prokaryotes' cytosol and in eukaryotes' Mitochondria to produce acetyl-CoA. The overall reaction in Beta oxidation is given below;
Cn-acyl-CoA + FAD + NAD^+ + H2O+ CoA ------------->Cn-2-acyl-CoA + FADH2 + NADH + H^+ + acetylCoA.
Beta-oxidation occurs by cleaving two carbons every cycle to form acetyl-CoA. It involves four process.
(1). Dehydrogenation of long chain fatty acids to create trans C2 and C3. This process is catalyzed by acyl CoA dehydrogenase.
(2). The hydration of Trans-delta2-enoyl CoA to produce L-3-hydroxyacyl CoA. This process is catalyzed by enoyl-CoA hydratase.
(3). The re-hydrogenation of L-3-hydroxyacyl CoA to produce 3-ketoacyl CoA in the presence of 3-hydroxyacyl CoA dehydrogenase.
(4). Thyolysis. In this process, thiolase enzyme catalyzes the reaction. A new molecule of coenzyme A breaks the bond by nucleophilic attack on C3.