Answer:
The correct answer is option A.
Explanation:
The separation of the two strands is not necessary for the replication process to occur. The initiation of the replication process is accomplished by the association with the origin of replication with the initiator proteins.
Prokaryotic cells consist only one DNA replication origin where is in the eukaryotic cells the replication is bidirectional and begins at multiple locations.
Thus, the correct answer is the option A)