Please choose the method of antimicrobial susceptibility testing that involves measuring and evaluating zones of inhibition around antibiotic discs placed on a culture of bacteria prior to incubation.A. Beta-lactamase testB. Tube dilution testC. Kirby-Bauer testD. Gram stain testE. ELISA test

Kirby-Bauer test is used to check the sensitivity of a particular bacteria towards an antimicrobial drug. So in this test, a pure culture of bacteria is isolated and its lawn is prepared on a suitable agar medium with the presence of discs of antibiotics that have known antibiotic amount.

Then the agar plate is incubated for appropriate time so that the bacteria can grow in the agar plate. After incubation, if the microbe is susceptible to antibiotic then a zone of inhibition will be seen where antibiotic growth is absent. More the zone of inhibition more will be the susceptibility of the bacteria towards that antibiotic.

So this test involves measuring and evaluating the zone of inhibition around antibiotic disc therefore the correct answer is C.